SequenceDigester

Will translate a FASTA file into a text file of peptides.

Features:

  • Use the whole genome or just the ORFs (Open Reading Frames)
  • Specify how many missed cleavages you want
  • Simulates Trypsin digestion
  • Set peptide mass criteria
  • Can use protein or DNA files
  • Removes duplicate peptides: peptides which have the same locus and same amino acid sequence except one terminates with a stop.